Research Associate I – Molecular Biology (IITA)

The position focuses on construct development and performing genomic DNA extractions, PCR, Southern blotting, qRT-PCR, sequencing, mutation detection, and maintaining bacterial cultures. The successful candidate will work closely with a multidisciplinary team of scientists and contribute to ongoing research in plant genome editing and trait enhancement.

Key Responsibilities

• Develop genome editing and RNAi constructs.
  
• Perform genomic DNA extraction from various transgenic and gene edited events of date palm, sugarcane or any other crops for molecular analysis.
  
• Conduct PCR (Polymerase Chain Reaction) and qRT-PCR (quantitative Real-Time PCR) to assess gene expression and mutation verification.
  
• Perform mutation detection assays, including T7 endonuclease and band-shift analysis.
  
• Carry out Southern blot analysis for gene copy number determination and transgene integration.
  
• Sequence analysis for detection and validation of gene edits using techniques such as Sanger or next-generation sequencing.
  
• Maintain and propagate bacterial cultures for plasmid DNA preparations.
  
• Contribute to the maintenance of gene-edited plant lines in the lab and greenhouse.
  
• Ensure accurate data collection, analysis, and record-keeping for experimental results.
  
• Assist in preparing reports, presentations, and manuscripts related to the research findings.
  
• Collaborate with other team members and provide technical support in molecular techniques.
  
• Perform any other duty assigned
  

Requirements

• Bachelor’s degree or diploma in molecular biology, Biotechnology, Genetics, or a related field.
  
• Relevant professional certification.
  
• Two years relevant experience with degree or four years’ relevant experience with diploma. Experience with in silico gene cloning tools such as Benchling and SnapGene
  
• Hands-on experience with molecular techniques such as DNA extraction, PCR, qRT-PCR, and sequencing.
  
• Familiarity with Southern blotting and mutation detection assays is a plus.
  
• Ability to maintain detailed lab records and follow protocols with precision.
  
• Strong organizational and time management skills.
  
• Excellent interpersonal and communication skills for working in a collaborative research environment.