Qualifications

· Bachelor’s degree in Microbiology, Medical Laboratory Science, Biology, Chemistry, Biochemistry, or related field.

· Master’s degree in a related discipline is an added advantage.

· Certification as a Technologist or Specialist in Microbiology is an advantage.

Experience

· 1–4 years’ experience in a stem cell laboratory setting.

· Proven expertise in stem cell isolation, differentiation, and characterisation.

· Experience with molecular biology techniques such as PCR, flow cytometry, and immunohistochemistry.

· Strong knowledge of stem cell biology, including pluripotent and adult stem cells.

Experimental Design and Execution

· Develop and implement experimental plans, including hypothesis development, protocol design, and project timelines.

· Optimise protocols for stem cell isolation, culture, expansion, differentiation, and maintenance.

· Conduct cellular reprogramming using advanced methodologies (e.g., Yamanaka factors, episomal vectors).

· Differentiate stem cells into specific cell types such as neurons, cardiomyocytes, and hepatocytes.

· Apply gene-editing technologies (e.g., CRISPR/Cas9) to advance functional studies.

· Perform immunocytochemistry, flow cytometry, and high-throughput assays to evaluate phenotypes.

Quality Assurance and Compliance

· Monitor cell morphology, proliferation, and viability to ensure consistent, high-quality stem cell cultures.

· Perform contamination checks, including routine mycoplasma testing.

· Adhere to Good Manufacturing Practice (GMP), FDA, and international ethical standards.

· Prepare and maintain Standard Operating Procedures (SOPs), risk assessments, and safety protocols.

Data Collection and Analysis

· Maintain accurate data using electronic laboratory notebooks and software.

· Perform statistical analysis to validate results and trends.

· Use bioinformatics tools for omics data analysis, including RNA-seq and single-cell sequencing.

· Prepare research reports, presentations, and visual data for internal and external stakeholders.

Collaboration and Communication

· Collaborate with clinicians, molecular biologists, and bioinformaticians on research projects.

· Participate in team meetings, cross-disciplinary initiatives, and grant applications.

· Contribute to manuscripts for peer-reviewed journals and present findings at conferences.

Laboratory Management and Mentorship

· Manage laboratory stocks, reagents, and equipment maintenance.

· Train and mentor junior staff, interns, and technicians in stem cell techniques.

· Provide quality assurance oversight for laboratory operations and experimental reproducibility.